Molecular Biology

The aim of this work is to integrate the gene for Green Fluorescent Protein (GFP) into the chromosomal DNA of Salmonella enteritidis. Our approach is to apply standard cloning techniques for the insertion of the PCR amplified GFP gene which is restricted from the Clontech™ plasmid (pGFP®) into an electrophoretically purified, enzymatically digested chromosomal fragment containing a selected non-coding region of the Salmonella enteritidis genome. The resulting recombinant DNA fragment will then be ligated to form a non-replicating 3.3 kb plasmid that is then amplified and inserted into Salmonella enteritidis for GFP integration using electroporation. 

The purpose of this experiment is to create a plasmid vector that contains an insert coding for green fluorescent protein (GFP) so that the gene for GFP can integrated into the chromosomal DNA of Salmonella enteritidis. Preliminary results obtained from E. coli strain K12 experiments suggest that integration is possible.